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Recently, the research group of Professor Li Wei, College of Food Science and Technology, Nanjing Agricultural University, together with Jiangsu Biodep Biotechnology Co.,Ltd. published a research paper titled "Effects of Lacticaseibacillus paracasei SNB-derived postbiotic components on intestinal barrier dysfunction and composition of gut microbiota" in Food Research International (Q1, IF = 8.1).
The first author of this paper is Xiao Luyao, a doctoral student in the College of Food Science and Technology, Nanjing Agricultural University, and Professor Li Wei is the corresponding author. This article focuses on the Probiotics-derived postbiotics and reveals the function and application potential of Lacticaseibacillus paracasei SNB-derived postbiotic components.These components protect against intestinal barrier damage, promote the proliferation of lactic acid bacteria as a major source of carbon and nitrogen, and regulate the intestinal flora positively.
Research background:
As we all know, intestinal flora is closely related to people's health, and many aspects of human physiological functions are affected by the complex intestinal microbiome, such as energy metabolism, intestinal barrier, immune and nervous systems. Considering the close relationship between intestinal flora and host health, dietary intervention is a common means to regulate intestinal flora. Probiotics are generally recognized as safe microorganisms. Currently, Lactobacillus and bifidobacterium are the main probiotic supplements, and the positive regulatory effects of probiotics on the host health have been fully studied.
According to the consensus statement issued by the International Scientific Association for Probiotics and Prebiotics (ISAPP), postbiotics are defined as "preparations of non-living microorganisms and/or their components that are beneficial to host health," including non-living bacteria, bacterial metabolites, and/or bacteria decomposing components. Postbiotics not only act as a symbol of human immunity regulation but also a nutrient that directly produces beneficial effects on human body. They have shown a unique effect superior to probiotics and prebiotics in regulating immune response, body metabolism, nerve conduction and other functions, and have made up for the shortcomings of probiotics in storage, transportation and processing with greater and wider potentials in application.
Lacticaseibacillus paracasei S-NB was isolated from traditional fermented milk in Aksu, Xinjiang, China by this lab. In previous studies, this strain showed advantages such as a high number of viable bacteria, rapid proliferation, and strong adhesion ability, etc. Whole-genome sequencing analysis, gene prediction and gene cluster analysis related to synthesis of surface macromolecules of this strain have been completed. Therefore, the study on the probiotic function evaluation of the surface components of S-NB strain and the regulation potential of intestinal flora will further promote the existing understanding of the potential functions of this strain. The objective of this study was to (1) investigate the protective mechanism of capsular polysaccharide (B-CPS) and surface protein (B-SLP) from Lacticaseibacillus paracasei S-NB derived postbiotics, on intestinal cell barrier damage; (2) evaluate whether B-CPS and B-SLP can be used as major carbon and nitrogen sources to promote the growth and proliferation of Lactobacillus of different species, respectively; (3) determine the effects of B-CPS and B-SLP on human intestinal microbial composition and SCFAs separately and unitedly.
Conclusion and Prospect:
In this study, the postbiotic components B-CPS and B-SLP were isolated from the surface of Lacticaseibacillus paracasei S-NB strain. First, an LPS-induced barrier damage model of Caco-2 intestinal epithelial cells was constructed, and it was confirmed that pretreatment with 100μg/mLB-CPS and B-SLP could increase the transmembrane resistance value of intestinal epithelial cells, enhance cell permeability, and increase the relative expression of tight junction protein. These results indicated that B-CPS and B-SLP were beneficial to repair the integrity of intestinal epithelial cells. In addition, it was found that the molecular weight of B-CPS and the particle size distribution of B-SLP did not change significantly after simulated gastrointestinal digestion in vitro, and B-CPS and B-SLP could serve as the main carbon and nitrogen sources, respectively, to promote the growth and proliferation of 8 different Lactobacillus strains. In vitro anaerobic fermentation experiments, B-CPS and the mixture of B-CPS and B-SLP in equal proportions could increase the abundance of beneficial intestinal flora, especially bifidobacterium, reduce the proportion of pathogens harmful to host, and promote the production of short-chain fatty acids. The correlation between environmental variables (pH, short-chain fatty acids) and the Top50 bacteria at the genus level was further analyzed, and the results showed that the level of short-chain fatty acids was positively correlated with the abundance of most beneficial bacteria. The results show that B-CPS and B-SLP, the postbiotic components of Lacticaseibacillus paracasei, have the potential to improve human intestinal health, and are expected to be further developed and applied in functional foods or health products.
About the author:
Li Wei, Professor, Associate Dean, doctoral Supervisor, College of Food Science and Technology, Nanjing Agricultural University; Visiting Scholar, Department of Medicine-GI/Nutrition, Harvard Medical School/Division Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Co-Supervisor: Wayne I. Lencer. He is also the deputy director of Jiangsu Province Agricultural Products Processing and Storage and Quality Control experimental teaching Demonstration Center, the secretary of the biological engineering teaching branch of Food Science and Technology College, and the head of Jiangyin Probiotic Expert Workstation of Nanjing Agricultural University. He is also a member of the third Youth Working Committee of the Chinese Society of Food Science and Technology, a director of the National Science and Technology Innovation Alliance for Food and Drug Homologous Industry, a vice chairman of the Nanjing Microbiology Society, and a member of the Youth Science and Technology Committee of Nanjing Agricultural University. He was selected as the third batch of "Zhong Shan Scholars Academic Rookie" Program of Nanjing Agricultural University (2015), "Excellent Young Backbone Teachers of Jiangsu Province Blue Project" (2016), and "Doctor of Double Innovation" (Vice President of Science and Technology) of Jiangsu Province High-level Innovation and Entrepreneurship Talent Introduction Program (2016). He was awarded the Outstanding Party Affairs Worker of Nanjing Agricultural University (2018), the "Most Beautiful Teacher" of Nanjing Southern Agricultural University (2019), and the Outstanding Contribution Award for Social Cooperation of Nanjing Agricultural University (2020). 2012-2015, 2020 unit assessment excellent, 2017 section level personnel recruitment period excellent assessment.Research areas:
1) microbial resource discovery and safety evaluation
2) Research and development of new prebiotics, probiotics and epigenetics
3) Studies on the immune activity of prebiotics, probiotics, and intestinal probiotics
4) Study on high density fermentation culture and activity preservation technology of active probiotics
5) Discovery of new resources of bacterial polysaccharides
6) Study on the function and activity of lactic acid bacteria polysaccharide.
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